LL37 Quality Identification: HPLC & Mass Spectrometry (CAS Antimicrobial peptide (sequence-specific))

Introduction

Verifying LL37 quality requires more than reading a headline purity number. This guide explains how B2B buyers should evaluate HPLC and mass spectrometry data for CAS Antimicrobial peptide (sequence-specific) (category: Immune, approx. MW 4493.2 Da) when procuring from Chinese peptide suppliers.

Expected Analytical Targets

• - Theoretical molecular weight reference: 4493.2 Da
• - CAS on supplier docs: Antimicrobial peptide (sequence-specific)
• - Research-grade HPLC purity commonly ≥98%
• - MS should show major ion consistent with sequence (+ salt form if acetate/TFA)

HPLC: What to Review

• - Full chromatogram image with integration
• - Column chemistry and gradient disclosed
• - Shoulder peaks and late eluters noted—not only main peak %
• - Batch number on report matches received vials

Mass Spectrometry: Identity Confirmation

For LL37, confirm the observed molecular ion matches calculated mass within instrument tolerance. Long peptides (4493.2 Da) may report average mass; clarify with supplier which convention is used.

Common MS Issues

• - Wrong adduct reported ([M+H]+ vs [M+Na]+)
• - Oxidized or dehydrated forms mistaken for main peak
• - Generic MS screenshot not tied to batch

When to Request Retesting

• - First order from new supplier
• - Assay failure despite high HPLC %
• - Visible cake discoloration or label mismatch
• - Publication or regulated program requirements

Supplier Qualification Workflow

• - Request two recent COAs for ${p.name}
• - Place minimum MOQ qualification order
• - Archive chromatogram PDFs in QMS
• - Add approved vendor before scale-up

Related catalog product: /products/ll37-5mg

Platform Disclaimer

PeptideFromChina.com is an informational directory. LL37 listings are for laboratory research and development only—not for human consumption or therapeutic use.

Need Help Sourcing?

Contact Peptide From China for manufacturer introductions supplying LL37 (CAS Antimicrobial peptide (sequence-specific)).