NAD+ Quality Identification: HPLC & Mass Spectrometry (CAS 53-84-9)

Introduction

Verifying NAD+ quality requires more than reading a headline purity number. This guide explains how B2B buyers should evaluate HPLC and mass spectrometry data for CAS 53-84-9 (category: Longevity, approx. MW 663.4 Da) when procuring from Chinese peptide suppliers.

Expected Analytical Targets

• - Theoretical molecular weight reference: 663.4 Da
• - CAS on supplier docs: 53-84-9
• - Research-grade HPLC purity commonly ≥98%
• - MS should show major ion consistent with sequence (+ salt form if acetate/TFA)

HPLC: What to Review

• - Full chromatogram image with integration
• - Column chemistry and gradient disclosed
• - Shoulder peaks and late eluters noted—not only main peak %
• - Batch number on report matches received vials

Mass Spectrometry: Identity Confirmation

For NAD+, confirm the observed molecular ion matches calculated mass within instrument tolerance. Long peptides (663.4 Da) may report average mass; clarify with supplier which convention is used.

Common MS Issues

• - Wrong adduct reported ([M+H]+ vs [M+Na]+)
• - Oxidized or dehydrated forms mistaken for main peak
• - Generic MS screenshot not tied to batch

When to Request Retesting

• - First order from new supplier
• - Assay failure despite high HPLC %
• - Visible cake discoloration or label mismatch
• - Publication or regulated program requirements

Supplier Qualification Workflow

• - Request two recent COAs for ${p.name}
• - Place minimum MOQ qualification order
• - Archive chromatogram PDFs in QMS
• - Add approved vendor before scale-up

Related catalog product: /products/nad-100mg-500mg

Platform Disclaimer

PeptideFromChina.com is an informational directory. NAD+ listings are for laboratory research and development only—not for human consumption or therapeutic use.

Need Help Sourcing?

Contact Peptide From China for manufacturer introductions supplying NAD+ (CAS 53-84-9).